Shameik Moore

Miles Morales / Spider-Man (voice)

Hailee Steinfeld

Gwen Stacy / Spider-Woman (voice)

Brian Tyree Henry

Jeff Morales (voice)

Luna Lauren Velez

Rio Morales (voice)

Oscar Isaac

Miguel O'Hara / Spider-Man 2099 (voice)

Jake Johnson

Peter B. Parker / Spider-Man (voice)

Jason Schwartzman

Jonathan Ohnn / Spot (voice)

Issa Rae

Jessica Drew / Spider-Woman (voice)

Daniel Kaluuya

Hobie Brown / Spider-Punk (voice)

Nicolas Cage

Peter Parker / Spider-Man Noir (voice)

John Mulaney

Peter Porker / Spider-Ham (voice)

Kimiko Glenn

Peni Parker (voice)

Amandla Stenberg

Margo Kess / Spider-Byte (voice)

Karan Soni

Pavitr Prabhakar / Spider-Man India (voice)

Mahershala Ali

Uncle Aaron (voice)

Jharrel Jerome

Miles G. Morales / Prowler (voice)

Greta Lee

LYLA (voice)

Andy Samberg

Ben Reilly / Scarlet Spider (voice)

Shea Whigham

George Stacy (voice)