Barrie Ingham

Basil of Baker Street / Bartholomew (voice)

Val Bettin

Dr. David Q. Dawson (voice)

Vincent Price

Professor Ratigan (voice)

Candy Candido

Fidget (voice)

Diana Chesney

Mrs. Judson (voice)

Eve Brenner

The Mouse Queen (voice)

Alan Young

Hiram Flaversham (voice)

Basil Rathbone

Sherlock Holmes (voice)

Ellen Fitzhugh

Bar Maid (voice)

Walker Edmiston

Citizen (voice)

Wayne Allwine

Thug Guard (voice)

Tony Anselmo

Thug Guard (voice)

Frank Welker

Toby the Dog / Felicia the Cat (voice) (uncredited)

Laurie Main

Dr. Watson (archive sound)

Shani Wallis

Lady Mouse (voice)

Melissa Manchester

Miss Kitty Mouse (voice)

Charles Fleischer

Additional Voices (voice) (uncredited)