Uma Thurman

Dr. Pamela Isley/Poison Ivy (voice)

Ralph Fiennes

Alfred Pennyworth (voice)

Fred Tatasciore

Bane (voice)

Maurice LaMarche

Mr. Freeze (voice)

Grey DeLisle

Vicki Vale and Jane (voice)

Frank Welker

Guard (voice)

John Glover

Dr Jason Woodrue (voice)