Troy Baker

Bruce Wayne / Batman / The Joker (voice)

Eric Bauza

Leonardo (voice)

Darren Criss

Raphael (voice)

Kyle Mooney

Michelangelo (voice)

Baron Vaughn

Donatello (voice)

Rachel Bloom

Barbara Gordon / Batgirl (voice)

Ben Giroux

Damian Wayne / Robin (voice)

Brian George

Alfred Pennyworth (voice)

Jim Meskimen

Comissioner Gordon / Jonathan Crane / Scarecrow (voice)

Tom Kenny

Oswald Chesterfield Cobblepot / The Penguin (voice)

John DiMaggio

Victor Fries / Mr. Freeze (voice)

Tara Strong

Harleen F. Quinzel / Harley Quinn / Pamela Isley / Poison Ivy (voice)

Carlos Alazraqui

Bane (voice)

Cas Anvar

Ra's al Ghul (voice)

Keith Ferguson

Harvey Dent / Two-Face / Baxter Stockman (voice)

Andrew Kishino

Shredder (voice)