Catherine O'Hara

Mrs. Frankenstein / Weird Girl / Gym Teacher (voice)

Martin Short

Mr. Frankenstein / Mr. Burgemeister / Nassor (voice)

Martin Landau

Mr. Rzykruski (voice)

Charlie Tahan

Victor Frankenstein (voice)

Atticus Shaffer

Edgar 'E' Gore (voice)

Winona Ryder

Elsa Van Helsing (voice)

Robert Capron

Bob (voice)

James Hiroyuki Liao

Toshiaki (voice)

Conchata Ferrell

Bob's Mom (voice)

Tom Kenny

New Holland Townsfolk (voice)

Frank Welker

Sparky (voice) (uncredited)

Dee Bradley Baker

Persephone / Shelly / Were-Rat / Colossus / Mr. Whiskers / Driver (voice) (uncredited)

Jeff Bennett

Giant Sea Monkeys (voice) (uncredited)